After three long months, I finally done it. I’ve extracted all of my roughly 180 RNA samples from my experiment. Let’s go. So today I’m gonna run through with you what I do in an RNA extraction. So right here, I’m homogenizing my tissue samples so that I get as much RNA as I possibly can, because I need. You don’t need a lot, but I want a lot, you know? Then after that, you add chloroform into your sample, and it does my favourite thing throughout the whole process, you have to shake it right here. And then afterwards, it looks like Pepto Bismol. By far the most exciting thing that’s gonna happen in this video. Oh, here it goes again. Watch. Unbelievably exciting. Then you toss that puppy into centrifuge and you get two different layers. The top is the aqueous, and that’s what I’m trying to extract. That’s where my RNA is. You can see me adding it into a separate test tube right here, and boom, I’ve extracted that surface layer. And now we have to toss that in a centrifuge with a little isopropanol, and boom, an RNA pellet. Very small, very tiny, very demure. And after that, you take it to the Gen 5 and you see how much RNA you extracted. Once you confirm that you have enough RNA and quality RNA, you then go to do gel electrophoresis to confirm again that it’s quality RNA. So just ran the gel and here You’ll see me add it onto UV light. And here comes another exciting part. You can actually see my two bands of RNA. Wow. Here are only a few of the samples I’ve extracted, but it feels good to be done. See ya. And, you know, I gotta hit a little fit check. This is what I wore today for the last extraction. Something simple, something plain for a little cutesy, you know? And before I let y’all go, quick little check on the paddle. One braid. Yes, sir.